教育经历

1996年9月-2000年7月，在北京大学化学与分子工程学院，本科生，获理学学士学位。

2000年9月-2005年7月，北京大学化学与分子工程学院，直读博士研究生，获分析化学博士学位。

工作经历

2005年9月-2009年7月，在加拿大阿尔伯塔大学临床医学和病理系分析和环境毒理学部，博士后。

2009年9月-2014年7月，山西大学环境科学研究所，教授。

2014年7月至今，威斯尼斯人wns888入口中国生态环境研究中心,环境化学与生态毒理学国家重点实验室，研究员。

承担科研项目：

1. 国家自然科学基金面上项目： 几种典型环境污染物小分子适配体的亲和性能表征及酶联变构适配体检测方法的构建和应用， 2019-2022，主持。

2. 威斯尼斯人wns888入口中国战略性先导专项B：污染物与生物分子交互作用机制，2014-2019，参加。

3. 威斯尼斯人wns888入口中国重点部署项目: 食品安全荧光检测技术与装备开发，2016，参加。

4. 国家自然科学基金面上项目: 基于适配体亲和分析生物毒素分子的研究，2016-2019，主持。

5. 国家自然科学基金重点项目：针对蛋白质-核酸相互作用研究的单分子荧光偏振分析方法学与技术，2015-2019，参加。

6. 国家自然科学基金优秀青年科学基金项目，分析化学，2013-2015，主持。

 获奖及荣誉：

1. 国家自然科学基金委优秀青年基金（2012年）

 代表性论文：

1. A Simple Aptamer Molecular Beacon Assay for Rapid Detection of Aflatoxin B1, Chao Wang, LinLin Sun, Qiang Zhao*, Chinese Chemical Letter, 2019, 30, 1017-1020.

2. Affinity Interactions by Capillary Electrophoresis: Binding, Separation, and Detection, Fangzhi Yu, Qiang Zhao, Dapeng Zhang, Zheng Yuan, Hailin Wang*, Analytical Chemistry, 2019, 91, 372–387

3. Aptamer-Structure Switch Coupled with Horseradish Peroxidase Labeling on a Microplate for the Sensitive Detection of Small Molecules, Yapiao Li, Linlin Sun, Qiang Zhao*, Analytical Chemistry, 2019, 91, 2615-2619

4. Nucleic Acid Aptamers Improving Fluorescence Anisotropy and Fluorescence Polarization Assays for Small Molecules, Qiang Zhao*, Jeffrey Tao, Jagdeesh S. Uppal, Hanyong Peng, Hailin Wang, X. Chris Le*, Trends in Analytical Chemistry, 2019, 110, 401-409.

5. A Simple Fluorescent Aptamer Based Assay Coupled with Fluorescence Scanning Capillary Array for Aflatoxin B1, Linlin Sun, Qiang Zhao*, Analyst, 2018, 143, 4600-4605.

6. Direct Fluorescence Anisotropy Approach for Aflatoxin B1 Detection and Affinity Binding Study by Using Single Tetramethylrhodamine Labeled Aptamer, Linlin Sun, Qiang Zhao*, Talanta, 2018, 189, 442-450.

7. Development of Aptamer Fluorescent Switch Assay for Aflatoxin B1 by Using Fluorescein-Labeled Aptamer and Black Hole Quencher 1-Labeled Complementary DNA, Yapiao Li, Linlin Sun, Qiang Zhao*, Analytical Bioanalytical Chemistry 2018, 410, 6269-6277.

8. Crystal Structures of Thrombin in Complex with Chemically Modified Thrombin DNA Aptamers Reveal the Origins of Enhanced Affinity, Rafal Dolot, Curtis H. Lam, Malgorzata Sierant, Qiang Zhao, Feng-Wu Liu, Barbara Nawrot, Martin Egli,* Xianbin Yang* Nucleic Acids Research 2018, 46, 4819-4830.

9. Competitive Horseradish Peroxidase-Linked Aptamer Assay for Sensitive Detection of Aflatoxin B1, Linlin Sun, Qiang Zhao*, Talanta, 2018, 179, 344-349.

10. Detection of Human Neutrophil Elastase by Aptamer Affinity Capillary Electrophoresis Coupled with Laser-induced Fluorescence Using Specified Site Fluorescently Labeled Aptamer, Yunlong Bai, Hailin Wang, Qiang Zhao*, Analytical Bioanalytical Chemistry , 2017, 409, 6843-6849.

11. Investigation of Effects of Metal Ions in Sample Buffer on Capillary Electrophoresis Coupled with Laser-Induced Fluorescence Analysis of Thrombin Using Dye-Labeled 29-mer DNA Aptamer, Yunlong Bai, Qiang Zhao*, Analytical Methods, 2017, 9, 5684-5690.

12. Enhancing the Affinity of Anti-Human α-Thrombin 15-mer DNA Aptamer and Anti-Immunoglobulin E Aptamer by PolyT Extension, Yunlong Bai, Yapiao Li, Dapeng Zhang, Hailin Wang, Qiang Zhao*, Analytical Chemistry, 2017, 89, 9467-9473.

13. Rapid Fluorescence Detection of Immunoglobulin E Using an Aptamer Switch Based on Binding-Induced Pyrene Excimer, Yunlong Bai, Qiang Zhao*, Analytical Methods, 2017, 9, 3962-3967.

14. Aptamer based surface plasmon resonance sensor for aflatoxin B1, Linlin Sun, Liqing Wu, Qiang Zhao*, Microchimica Acta, 2017, 184, 2605-2610.

15. Competitive fluorescence anisotropy/polarization assay for ATP using aptamer as affinity ligand and dye-labeled ATP as fluorescence tracer, Yapiao Li, Linlin Sun, Qiang Zhao*, Talanta, 2017, 174, 7-13.

16. Direct fluorescence anisotropy assay for cocaine using tetramethylrhodamine-labeled aptamer, Yingxiong Liu, Qiang Zhao*, Analytical Bioanalytical Chemistry, 2017, 409, 3993-4000.

17. Affinity capillary electrophoresis with laser induced fluorescence detection for thrombin analysis using nuclease-resistant RNA aptamers, Lihua Hao, Yunlong Bai, Hailin Wang, Qiang Zhao* , Journal of Chromatography A, 2016, 1476, 124-129.

18. Determination of the platelet-derived growth factor BB by a competitive thrombin-linked aptamer-based fluorometric assay, Limin Guo, Qiang Zhao*, Microchimica Acta, 2016, 183, 3229-3235.

19. An aptamer assay using rolling circle amplification coupled with thrombin catalysis for protein detection, Limin Guo, Lihua Hao, Qiang Zhao*, Analytical Bioanalytical Chemistry, 2016, 408, 4715-4722.

20. Thrombin-linked aptamer assay for detection of platelet derived growth factor BB on magnetic beads in a sandwich format, Limin Guo, Qiang Zhao*, Talanta, 2016, 158, 159-164.

21. Microplate based assay for thrombin detection using an RNA aptamer as affinity ligand and cleavage of a chromogenic or a fluorogenic peptide substrate, Lihua Hao, Qiang Zhao*, Microchimica Acta, 2016, 183, 1891-1898.

22. Fluorescence Anisotropy Assay for D-Vasopressin with Tetramethylrhodamine-Labeled Aptamer, Yingxiong Liu, Qiang Zhao*, Analytical Methods, 2016, 8, 2383-2390.

23. Using Fluoro Modified RNA Aptamers as Affinity Ligands on Magnetic Beads for Sensitive Thrombin Detection through Affinity Capture and Thrombin Catalysis, Lihua Hao, Qiang Zhao*, Analytical Methods, 2016, 8, 510-516.

24. Nanoparticles-Free Fluorescence Anisotropy Amplification Assay for Detection of RNA Nucleotide-Cleaving DNAzyme Activity, Dapeng Zhang, Rong Fu, Qiang Zhao, Haiqin Rong, Hailin Wang*, Analytical Chemistry, 2015, 87, 4903-4909.

25. A Fluorescein Labeled Aptamer Switch for Thrombin with Fluorescence Decrease Response, Lihua Hao, Qiang Zhao*, Analytical Methods, 2015, 7, 3888-3892.

26. Aptamer and Rolling Circle Amplification-Involved Sandwich Assay for Platelet-Derived Growth Factor-BB with Absorbance Analysis, Li Lv, Limin Guo, Qiang Zhao*, Analytical Methods, 2015, 7, 1855-1859.

27. Aptamer Fluorescence Anisotropy Sensors for Adenosine Triphosphate by Comprehensive Screening Tetramethylrhodamine Labeled Nucleotides, Qiang Zhao*, Qin Lv, Hailin Wang, Biosensors Bioelectronics, 2015, 70, 188-193.

28. Sensitive and Selective Detection of Thrombin by Using a Cyclic Peptide as Affinity Ligand, Qiang Zhao*, Jie Gao, Biosensors Bioelectronics, 2015, 63, 21-25.

29. Identification of Allosteric Nucleotide Sites of Tetramethylrhodamine-Labeled Aptamer for Noncompetitive Aptamer-Based Fluorescence Anisotropy Detection of a Small Molecule, Ochratoxin A, Qiang Zhao*, Qin Lv, Hailin Wang, Analytical Chemistry, 2014, 86, 1238-1245.

30. An Affinity Capture Involved Enzymatic Assay for Thrombin by Using Peptide Aptamers as Affinity Ligands on Magnetic Beads, Qiang Zhao*, Jie Gao, Chemical Communications, 2013, 49, 7720-7722.

31. Detection of Thrombin Using Excimer Aptamer Switch Labeled with Dual Pyrene Molecules, Lin Cheng, Qiang Zhao*, Analytical Bioanalytical Chemistry, 2013, 405, 8233-8239.

32. Screening Interaction between Ochratoxin A and Aptamers by Fluorescence Anisotropy Approach. Xia Geng, Dapeng Zhang, Hailin Wang, Qiang Zhao*, Analytical Bioanalytical Chemistry, 2013, 405, 2443-2449.

33. Specific and Sensitive Fluorescence Anisotropy Sensing of Guanine-Quadruplex Structures via a Photoinduced Electron Transfer Mechanism, Dapeng Zhang, Hujun Shen, Guohui Li, Bailin Zhao, Anchi Yu, Qiang Zhao, Hailin Wang*, Analytical Chemistry, 2012, 84, 8088-8094.

34. Fluorescence Anisotropy Reduction of Allosteric Aptamer for Sensitive and Specific Protein Signaling, Dapeng Zhang, Qiang Zhao, Bailin Zhao, Hailin Wang*, Analytical Chemistry, 2012, 84, 3070-3074.

35. Applications of Aptamer Affinity Chromatography, Qiang Zhao*, Minghuo Wu, X. Chris Le, Xing-Fang Li*, Trends in Analytical Chemistry, 2012, 41, 46-57.

36. An Aptamer-Capture Based Chromogenic Assay for Thrombin. Qiang Zhao,* Xiaofang Wang, Biosensors Bioelectronics, 2012, 34, 232-237.

37. Aptamer Capturing of Enzymes on Magnetic Beads to Enhance Assay Specificity and Sensitivity. Qiang Zhao*, Xing-Fang Li, X. Chris Le, Analytical Chemistry, 2011, 83, 9234-9236.

38. Detection of Escherichia coli O157:H7 Using Gold Nanoparticle Labeling and Inductively Coupled Plasma-Mass Spectrometry. Feng Li, Qiang Zhao, Xiufen Lu, Xing-Fang Li, X. Chris Le*, Analytical Chemistry, 2010, 82, 3399-3403.

39. Aptamer-Linked Assay for Thrombin Using Gold Nanoparticle Amplification and Inductively Coupled Plasma Mass Spectrometry Detection. Qiang Zhao, Xiufen Lu, Chun-gang Yuan, Xing-Fang Li, X. Chris Le*, Analytical Chemistry, 2009, 81, 7484-7489.

40. Aptamer-Based Affinity Chromatographic Assay for Thrombin. Qiang Zhao, Xing-Fang Li, Yuanhua Shao, X. Chris Le*, Analytical Chemistry, 2008, 80, 7586-7593.

41. Aptamer-Modified Monolithic Capillary Chromatography for Protein Separation and Detection. Qiang Zhao, Xing-Fang Li, X. Chris Le*, Analytical Chemistry, 2008, 80, 3915-3920.